ABSTRACT
Objective:To analyze the changes of chemical compositions and relative contents of volatile constituents in raw Xanthi-um sibiricum Patr. and fried Xanthium sibiricum Patr. Methods: Headspace solid phase micro extraction ( HS-SPME) combined with gas chromatography-mass spectrometry ( GC-MS) was used to study the volatile components in raw and fried products of Xanthium sibir-icum Patr. . A peak area normalization method was applied to calculate the relative percentage content of each composition. Results:Totally 26 and 27 kinds of compounds were isolated and identified respectively from raw and fried xanthium including 19 species and re-spectively accounting for 68. 69% and 90. 08% of the total volatile components. In the raw products, the components with high con-tents were camphor (15. 3%), eucalyptus oil alcohol (9. 6%) and borneol (6. 89%), while in the fried products, those were men-thol (24. 09%), borneol (18. 48%) and eucalyptol (7. 19%). Conclusion:The relative contents of components with effects on rhi-nitis in the fried products are obviously higher than those in the raw products. HS-SPME-GC-MS can be used to detect the volatile com-positions and their relative contents in the raw products and fried products of Xanthium sibiricum Patr. . From the changes of volatile components, theoretical basis can be laid for the components with certain efficacy in raw and fried Xanthium sibiricum Patr. , and scien-tific basis can be provided for their further study and comprehensive utilization.
ABSTRACT
Objective:To compare and analyze the volatile components in different medicinal parts of silktree Albizzia julibrissin and their relative contents .Methods:Chromatography-mass spectrometry using headspace solid phase microextraction combined with gas method ( HS-SPME-GC-MS) was used to analyze the volatile components in the bark and flowers of silktree Albizzia julibrissin and the relative contents were calculated by a peak area normalization method .Results:In the bark and flowers of silktree Albizzia julibris-sin, totally 30 and 33 compounds were respectively identified , which respectively accounted for 73.20%and 88.29%of the total vola-tile.A total of 15 components, including alkanes, alkenes, aldehydes, furans, alcohols and acids were in the bark and flowers of silk-tree Albizzia julibrissin, among them, the content of cineole was the highest (bark:15.02%and flowers:21.9%).Conclusion:The HS-SPME technique can be used to analyze and compare the volatile components in the bark and flowers of silktree Albizzia julibrissin, which provides scientific basis for the reasonable development and application of the bark and flowers of silktree Albizzia julibrissin.
ABSTRACT
Objective:To comparatively study the chemical constituents in n-butanol part from Magnoliae Officinalis cortex before and after ginger mix-frying by HPLC-MS. Methods:A 4000 Q TRAP MS system was used with a C18 chromatographic column. Metha-nol-water with gradient elution was employed as the mobile phase. The data were collected by an electrospray ion source under the mode of positive and negative ions. The chemical constituents were analyzed by contrasting with the reference substances, analyzing the mass spectrometry data and retrieving literatures. Results:The negative ion mode had better separation for n-butanol part, and fourteen components with known peaks and the other unknown compositions were detected. The positive ion mode could detect fewer peaks, and the detected [ M+H] + peaks malnly were hydrogenation peaksmalnly for phenolic constituents. Conclusion:Through the analysis and comparison, it is suggested that n-butanol part from Magnoliae Officinalis cortex has qualitative and quantitative changes after ginger mix-frying.